Enzyme-linked immunosorbent assay (ELISA)
Cell culture supernatants were collected following PGD2stimulation (24 hours) and cytokine concentration determined using ELISA for IL-5 and IL-13 according to the manufacturer’s instructions. Samples were measured in triplicate and were all within the range of the standard curve.

Statistical Analysis

Descriptive data for clinical characteristics between men and women and between mild/moderate and severe asthma are reported using proportions for dichotomous variables or means (Min-Max) for continuous variables, as appropriate. Bivariable comparisons of dichotomous variables are performed using chi-squared (χ2) test whereas Mann-Whitney U test are performed for continuous variables. Statistical analyses were performed using SPSS (version 21.1, Chicago, IL, USA) and R (R Team, 2020). As multiple statistical tests were performed on the clinical data, P -values less than 0.01 were considered statistically significant. For in vitro experiments with Th2 cells statistical significance between conditions was determined using One Way Repeated Measures (RM) ANOVA with Student-Newman-Keuls for multiple comparisons. P -values less than 0.05 were considered significant.