Enzyme-linked immunosorbent assay (ELISA)
Cell culture supernatants were collected following PGD2stimulation (24 hours) and cytokine concentration determined using ELISA
for IL-5 and IL-13 according to the manufacturer’s instructions. Samples
were measured in triplicate and were all within the range of the
standard curve.
Statistical Analysis
Descriptive data for clinical characteristics between men and women and
between mild/moderate and severe asthma are reported using proportions
for dichotomous variables or means (Min-Max) for continuous variables,
as appropriate. Bivariable comparisons of dichotomous variables are
performed using chi-squared (χ2) test whereas
Mann-Whitney U test are performed for continuous variables. Statistical
analyses were performed using SPSS (version 21.1, Chicago, IL, USA) and
R (R Team, 2020). As multiple statistical tests were performed on the
clinical data, P -values less than 0.01 were considered
statistically significant. For in vitro experiments with Th2
cells statistical significance between conditions was determined using
One Way Repeated Measures (RM) ANOVA with Student-Newman-Keuls for
multiple comparisons. P -values less than 0.05 were considered
significant.