Figure S3: When omitting the class Actinopterygii, observed, Faith’s phylogenetic, Shannon and Simpson alpha diversity indexes fromM. festivus gut samples (n = 40) amplified using different blocking primer combinations (n = 5). We clustered and colored samples according to their blocking primer group. The plot was produced using the function plot_richness from Phyloseq . There was a significant difference between the phylogenetic diversity from the M_2X group and the control (p-value < 0.05), but there was no significant difference in Shannon and Simpson diversity indexes (p-value > 0.9).