3.3 Combination of two-step crosslinking approach
Two-step crosslinking resulted in substantial cohesion. Figure 3A showed
that 10 s pre-crosslinked GelMA can be re-crosslinked with uncrosslinked
GelMA bioink and formed a united combination. There was no sign of
splitting at the combining site under the SEM observation (Figure 3b).
In tensile loading, there was no significant difference in Young’s
modulus and tensile strain between structures formed by one-step or
two-step crosslinking methods (P >0.05) (Figure
3C-3D).