Bioprinting small-diameter vascular vessel with endothelium and smooth muscle by the approach of two-step crosslinking process:Sacrificial hydrogels are used as temporary lumen support to mold tubular structure which will affect the structure’s stability. To avoid using the sacrificial hydrogel, we develop a new bioprinting approach to fabricating small-diameter vessel using two-step crosslinking process. Bioprinting the GelMA-based hydrogel containing Smooth muscle cell (SMCs) and perfused with human umbilical vein endothelial cells (HUVECs), a complicated bionic vascular vessel with dual layers is fabricated.