Figure 11. Reusability of MTCFs@SIP@CBMA and MTCFs@NIP@CBMA (a), SEM
(b), TEM images (c), and VSM curves (d) of MTCFs@SIP@CBMA after 8
cycles. Error bars represent ± standard deviations, n = 3.
Conclusion
Dedicated to the construction of
high-performance imprinted materials, this work developed an
anti-protein adsorption segment CBMA-assisted self-driven BSA surface
imprinted magnetic tubular carbon nanofibers MTCFs@SIP@CBMA with high
recognition selectivity using a strategy of combining magnetic
nanomaterial surface imprinting technique with amine-Michael addition.
The carrier is magnetic tubular carbon nanofibers (MTCFs) prepared by
hypercrosslinking polymerization and vacuum high-temperature
carbonization. The special structure of MTCFs including porous tube
wall, the cavity in the tube and the iron nanoparticles loaded in the
tube endows MTCFs@SIP@CBMA with magnetic performance and self-driven
adsorption performance, which simplifies the separation process while
improving the adsorption capacity and speeding up the adsorption rate.
The adsorption kinetics study confirms that MTCFs@SIP@CBMA exhibits
significant self-driven adsorption characteristics. The saturated
adsorption capacity is 395.26 mg/g with an imprinting factor of 6.88. To
improve the recognition selectivity of imprinted materials on the
premise of ensuring high adsorption capacity, biofriendly dopamine is
selected as a functional monomer for the coating of imprinted layer. At
the same time, the imprinted surface is grafted with CBMA polymer chain
by the method of amino-Michael addition to reduce non-specific
adsorption. This method makes the length and position of the CBMA
polymer chain controllable. When
WBSA-MTCFs@SIP/WCBMA was 1:10, the
length of CBMA polymer chain is the most suitable. Correspondingly,
MTCFs@SIP@CBMA possesses the strongest anti-nonspecific adsorption
effect, which in turn demonstrates the highest imprinting factor for
BSA. Due to the contribution of anti-protein adsorption chain segments,
MTCFs@SIP@CBMA presents excellent ability to specifically recognize BSA
in both competitive adsorption and actual sample separation experiments.
Furthermore, the adsorption capacity of MTCFs@SIP@CBMA lose only 4.76%
after eight times of repeated adsorptions-desorption, indicating its
excellent reusability. All in all, the high-performance protein
imprinted material MTCFs@SIP@CBMA provides potential practical
application value and is expected to play a role in the development of
protein drugs and drug diagnosis.