Figure 1 Synthesis schematic diagram of MTCFs@SIP@CBMA
Influence of CBMA polymer chain length on the imprinting factor of
MTCFs@SIP@CBMA
The strength of the anti-protein effect depended on the length of the
CBMA polymer segments. Meanwhile, the length of the CBMA polymer
segments could be intuitively reflected by the thickness of the CBMA
polymer layer. The length of the CBMA anti-protein adsorption chain was
controlled by changing the amount of CBMA added during the amino-Michael
addition process. As shown in Figure 2a, the thickness of the CBMA layer
gradually increased as the dose of CBMA increased, indicating that the
length of the CBMA polymer chain gradually increased. The effect of CBMA
polymer chain length on the adsorption capacity and imprinting factor of
MTCFs@SIP@CBMA is shown in Figure 2b. It can be seen that with the
increase of CBMA polymer chain length, the adsorption capacity of
MTCFs@SIP@CBMA on BSA gradually decreased, while the imprinting factor
demonstrated a trend of first increasing and then decreasing. This was
attributed
to the weak anti-protein effect when the CBMA chain length was too
short. So, the non-specific adsorption of the materials for non-template
proteins cannot be effectively reduced. However, too long CBMA chains
could easily lead to strong anti-protein adsorption effect and increase
steric hindrance, which was not conducive to the binding of template
proteins to imprinting sites. The experimental results suggested that
when the mass ratio of BSA-MTCFs@SIP to CBMA was 1:10, the CBMA chain
length was the most appropriate. MTCFs@SIP@CBMA possessed the highest
imprinting factor for BSA. Therefore, MTCFs@SIP@CBMA prepared at this
ratio was employed in the subsequent experiments.
According to the results obtained,
to significantly improve the recognition selectivity of imprinted
materials, it was necessary to ensure that the imprinting sites were not
severely affected by the CBMA anti-protein adsorption segments, while
the effect of anti-protein adsorption chain segments reducing
non-specific adsorption should be maximized by optimizing the chain
length of CBMA polymers.