Figure 9 UV spectra of binary solution of BSA-Bhb on MTCFs@SIP@CBMA and MTCFs@NIP@CBMA (a); SDS-PAGE analysis of adsorption for BSA by MTCFs@SIP@CBMA from protein mixture (b). Lane 1: marker; lane 2: protein mixed solution; lane 3: remaining protein mixture after adsorption by MTCFs@SIP@CBMA; lane 4: remaining protein mixture after adsorption by MTCFs@NIP@CBMA; lane 5: the eluent from MTCFs@SIP@CBMA; The dosage of protein solution was 10 μL.
Real sample analysis
Selecting fetal bovine serum (FBS) as a complex biological sample, the ability of MTCFs@SIP@CBMA to selectively isolate BSA from real samples was investigated. The results of UV spectra and SDS-PAGE gel electrophoresis analysis of the 30-fold diluted FBS stock solution, protein supernatants after adsorption by MTCFs@SIP@CBMA and MTCFs@NIP@CBMA, and the primary eluent of MTCFs@SIP@CBMA are shown in Figure 10. As observed from Figure 10a, the absorbance of FBS at 278 nm was significantly reduced after adsorption by MTCFs@SIP@CBMA. At the same time, the eluate of MTCFs@SIP@CBMA only appeared an absorption peak at 278 nm attributable to BSA, indicating that MTCFs@SIP@CBMA could specifically bind BSA from FBS. Furthermore, only a BSA band appeared in lane 5 of the corresponding electrophoretic photo (Figure. 10b), confirming again that MTCFs@SIP@CBMA can selectively isolate and capture BSA from real FBS. As a result, MTCFs@SIP@CBMA was expected to achieve the separation and enrichment of BSA in complex biological samples.