Figure 9 UV spectra of binary solution of BSA-Bhb on
MTCFs@SIP@CBMA and MTCFs@NIP@CBMA
(a); SDS-PAGE analysis of adsorption for BSA by MTCFs@SIP@CBMA from
protein mixture (b). Lane 1:
marker;
lane 2: protein mixed solution;
lane 3: remaining protein mixture after adsorption by MTCFs@SIP@CBMA;
lane 4: remaining protein mixture after adsorption by MTCFs@NIP@CBMA;
lane 5: the eluent from MTCFs@SIP@CBMA; The dosage of protein solution
was 10 μL.
Real sample analysis
Selecting fetal bovine serum (FBS) as a complex biological sample, the
ability of MTCFs@SIP@CBMA to selectively isolate BSA from real samples
was investigated. The results of UV spectra and SDS-PAGE gel
electrophoresis analysis of the 30-fold diluted FBS stock solution,
protein supernatants after adsorption by MTCFs@SIP@CBMA and
MTCFs@NIP@CBMA, and the primary eluent of MTCFs@SIP@CBMA are shown in
Figure 10. As observed from Figure 10a, the absorbance of FBS at 278 nm
was significantly reduced after adsorption by MTCFs@SIP@CBMA. At the
same time, the eluate of MTCFs@SIP@CBMA only appeared an absorption peak
at 278 nm attributable to BSA, indicating that MTCFs@SIP@CBMA could
specifically bind BSA from FBS. Furthermore, only a BSA band appeared in
lane 5 of the corresponding electrophoretic photo (Figure. 10b),
confirming again that MTCFs@SIP@CBMA can selectively isolate and capture
BSA from real FBS. As a result, MTCFs@SIP@CBMA was expected to achieve
the separation and enrichment of BSA in complex biological samples.