2.1 Gene and Protein Expression analysis
Expression of the IRX4 gene has been well established and identified in different tissues at the RNA level by RNA seq data. There is yet a gap in determining the expression of IRX4 at the protein level. To fill this hole, we initially searched different databases to identify the presence of IRX4 protein in healthy tissues and disease states. Proteomics database (ProteomicsDB) contains quantitative mass spectrometry data for most human proteins37. Next, we used PaxDb (Protein Abundances across organisms), a database to identify protein abundance at the proteome-wide level38. Protein quantification was reported as parts per million (ppm), describing the protein quantification with reference to the entire proteome.