3.4 Soil fungi community composition
Fungi were clustered at the phylum level. The histogram of community structure constructed according to OTU sequence abundance after clustering (Fig. 4c–d) revealed structural and abundance differences among N application rates and tillage treatments. There were five phyla of eumycota with an abundance > 0.01% in these treatments. Ascomycota and Mortierellomycota were the two most dominant, accounting for > 60% of all phyla.
N application, tillage, and soil depth significantly affected the fungal ITS community composition (Table S5). The abundance of Basidiomycota was higher under NT than under CT and showed a trend of first decreasing, then increasing with increasing N application rates under NT. We also found that the relative abundance of Mortierellomycota was higher under CT than NT in both layers.