3.4 Soil fungi community composition
Fungi were clustered at the phylum level. The histogram of community
structure constructed according to OTU sequence abundance after
clustering (Fig. 4c–d) revealed structural and abundance differences
among N application rates and tillage treatments. There were five phyla
of eumycota with an abundance > 0.01% in these treatments.
Ascomycota and Mortierellomycota were the two most dominant, accounting
for > 60% of all phyla.
N application, tillage, and soil depth significantly affected the fungal
ITS community composition (Table S5). The abundance of Basidiomycota was
higher under NT than under CT and showed a trend of first decreasing,
then increasing with increasing N application rates under NT. We also
found that the relative abundance of Mortierellomycota was higher under
CT than NT in both layers.