African swine fever virus propagation and enrichment:
The two ASFV isolates IND/AR/SD-61/2020 and IND/AS/SD-02/2020propagated
in porcine monocyte culture showed specific adsorption of porcine
erythrocytes around the infected monocytes as rosette (Fig.1) from 48
hours post infection. The concentration of genomic DNA extracted from
enriched viral pellets was 9.6ng/µL and 11.2ng/µL with A260/280 ratio of
around 1.8 and 1.7, respectively, for the two isolates.