African swine fever virus propagation and enrichment:
The two ASFV isolates IND/AR/SD-61/2020 and IND/AS/SD-02/2020propagated in porcine monocyte culture showed specific adsorption of porcine erythrocytes around the infected monocytes as rosette (Fig.1) from 48 hours post infection. The concentration of genomic DNA extracted from enriched viral pellets was 9.6ng/µL and 11.2ng/µL with A260/280 ratio of around 1.8 and 1.7, respectively, for the two isolates.