Pollen DNA metabarcoding and related methods in global change ecology: prospects, challenges, and progress
Karen L. Bell1,a, Katherine J. Turo2, Abigail Lowe3,b, Kevin Nota4,b, Alexander Keller5,b, Francisco Encinas-Viso6,b, Laura Parducci3,7,b, Rodney Richardson8,b, Richard M. Leggett9, Berry J. Brosi10, Kevin S. Burgess11, Yoshihisa Suyama12, Natasha de Vere13
1 CSIRO Health & Biosecurity and CSIRO Land & Water, 147 Underwood Avenue, Floreat, WA, Australia; School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia.
2 Department of Ecology, Evolution, and Natural Resources, Rutgers University, New Brunswick, NJ, 08901, United States.
3 National Botanic Garden of Wales, Llanarthne, United Kingdom.
4 Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, 75236 Uppsala, Sweden.
5 Organismic and Cellular Interactions, Biocenter, Ludwig-Maximilians-Universität München, Großhaderner Str. 2, 82152 Planegg, Germany.
6Centre for Australian National Biodiversity Research, CSIRO, Black Mountain, ACT, Australia
7Department of Environmental Biology, Sapienza University of Rome, Rome, Piazzale Aldo Moro 5, 00185, Italy.
8 Appalachian Laboratory, University of Maryland Center for Environmental Science, 301 Braddock Road, Frostburg, MD 21532, United States.
9 Earlham Institute, Norwich Research Park, Norwich, Norfolk, NR4 7UZ, United Kingdom.
10 Department of Biology, University of Washington, USA
11 Department of Biology, College of Letters and Sciences, Columbus State University, University System of Georgia, United States.
12 Field Science Center, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi 989-6711, JAPAN
13 Natural History Museum of Denmark, University of Copenhagen, Denmark
a Corresponding author: karen.bell@csiro.au
b Authors AL, KN, AK, FEV, LP, and RR all contributed equally.
ORCID:
KLB: 0000-0002-4445-4026
KJT: 0000-0003-1578-4592
AL: 0000-0001-5431-8936
KN: 0000-0002-4744-5205
AK: 0000-0001-5716-3634
FEV: 0000-0003-0426-2342
LP: 0000-0003-1956-4757
LP: 0000-0003-1956-4757
YS: 0000-0002-3136-5489
NdV: 0000-0001-9593-6925
Short running title: Pollen metabarcoding in global change ecology