Molecular identification of Hyalomma ticks
Hyalomma specimens were homogenised with Bertin Minilys (Bertin Instruments, Montigny-le-Bretonneux, France) after 3 times freeze-thawing in liquid nitrogen. We used multiple beads in various sizes with the addition of quartz sand after the freeze-thaw cycles and before proceeding to . .Samples were sequenced at a commercial centre (Eurofins Genomics, Ebersberg, Germany).
Sequence data were subjected for phylogenetic analysis, using the MEGA11 (Tamura, Stecher, & Kumar, 2021). The visualisation of final data was performed with iTOL v6 online tool.