Fig. 2. Metabolic pathway engineering for TAL biosynthesis inR. toruloides . PDC1, pyruvate decarboxylase; ALD5, acetylaldehyde
dehydrogenase; ACS1, acetyl-CoA synthetase ACS1, ACC1, acetyl-CoA
carboxylase; GhPS, 2-pyrone synthase gene from Gerbera hybrida ;
PDH, pyruvate dehydrogenase; ACL1, citrate lyase; YLACL1, citrate lyase
from Yarrowia lipolytica ; AMPD1, AMP deaminase; ME1, malic
enzyme; PEX10, peroxisomal matrix protein; PYC1, pyruvate carboxylase;
MLS1, cytosolic malate synthase; CIT2, peroxisomal citrate synthase;
DGA1, diacylglycerol acyltransferase; LRO1, lecithin cholesterol
acyltransferase. Note: Some metabolites were not positioned following
their intracellular compartmentalization.