Figure 3: Effect of different linkers on activity of the AadA-CodA fusion protein. (A ) C-A.f, C A.r and C-A.s possess a flexible, rigid and short linker, respectively. (B ) Biolistic transformation efficiencies obtained with each construct. (C ) PCR confirmation of plastome homoplasmy with the untransformed WT strain giving a 2455 bp product which is absent from a representative of each of the three transformant lines. Conversely, the three lines yield a 2078 bp product diagnostic of the transgenic plastome. (D ) Spot tests of mid-log cultures and 1:10 and 1:100 dilutions on TAP plates containing: no antibiotic, Spc or 5-FC.