Targeted sequencing
The Ion AmpliSeq Custom Panel of the 21 genes involved in the
PI3K/AKT/mTOR pathway (i.e., PIK3R1, PIK3R2, PIK3CA, PTEN, PDK1,
PDK2, KRAS, AKT1, AKT2, AKT3, RICTOR, MAPKAP1, MLST8, MTOR, IRS1, GAB1,
GAB2, THEM4, MAPK8I1, PTPN11 , and RAPTOR ) was used according to
our previous report. (Loconte DC et al 2015, PlosOne).