Sample collection
Eight wild boar livers (50 mg each) previously found to be positive for
HEV-3 by real-time RT-PCR in previous studies (De Sabato et al., 2018a;
Di Pasquale et al., 2019; De Sabato et al., 2020a; Pierini et al., 2021)
were used in the present study (Table 1). Originally, the livers were
obtained from adult wild boars hunted in the region of Lazio (province
of Viterbo; North Lazio) in 2016 and 2017 and in the neighbouring region
of Umbria (Perugia province) in 2018. RNA was extracted from liver using
the Qiamp-Viral mini kit (Qiagen) as previously described (De Sabato et
al., 2018b).