Sequence Analysis
22 nucleotide mutations result in nonsynonymous substitutions were identified (Table 2). All CPV-2 strains presented residue mutations at Phe267Tyr, and Tyr324Ile. All New CPV-2a strains carried a specific mutation Thr440Ala, and all CPV-2c strains exhibited unique mutation Ala5Gly and Gln370Arg while one New CPV-2a strain also harboured the mutation Ala5Gly. Mutation at residue Pro13Ser presented in two CPV-2c strains and one New CPV-2a strain. Another mutation found was Lys570Arg in one CPV-2c strain. Mutation Ile101Thr was observed in two FPLV strains and Pro238Gln in one FPLV strain.
Table 2Amino acid variations of CPV-2 strains in this study compared with reference strains