2.5.1. Fasting blood glucose and fasting insulin measurements
At week 2 and 16 all mice were fasted overnight and blood were taken from their tails in order to measure their fasting blood glucose and insulin levels (Zhang et al., 2008). Fasting blood glucose was measured with a One Touch II glucose meter (Lifescan, Inc., Johnson & Johnson, Milpitas, CA). Fasting insulin concentrations were measured in duplicates using the enzyme-linked immunosorbent assay (insulin ELISA, # nr 10-1247-01, Mercodia, Sweden) and the spectrophotometric plate reader (Synergy HT Multi-Mode Microplate Reader, BioTek, UK).