2.4. Experimental design
Mice were randomly classified into 3 groups (n=7 each) one group served
as a control nondiabetic group and two groups were injected with
streptozotocin (45mg/kg, intraperitoneally) to induce diabetes, then the
three groups received their respective drug treatments daily for 16
consecutive weeks according to the following design:
• Group 1; control non-diabetic mice treated with distilled water orally
and citrate buffer intraperitoneally.
• Group 2; nontreated induced diabetic mice.
• Group 3; induced diabetic mice treated with vitamin A (4- IU/ day
orally) as suggested by (Beck and Spiegelberg, 1989). At
2nd and 16th weeks, the levels of
fasting blood glucose, fasting insulin levels, lipid profile were
examined. After 16 weeks, free radicals and antioxidants were examined.