2.5.5. Evaluation of antioxidant enzymes
SOD activity in the supernatant was measured according to the
method suggested by (Nishikimi et al.,
1972). Results were defined as units per milligram protein (U/mg
protein).
CAT activity in the supernatant was determined according to the
method described by Aebi (1984) Results
were reported as the rate constant per second per milligram protein
(k/mg protein). Measurements of the decomposition of hydrogen peroxide
(H2O2) were performed
spectrophotometrically according to the method suggested
by Aebi
(1984) in the UV area of the light spectrum (240 nm, reading every 0.1
sec for 65 sec) using Lambda Software for data computation.
GPO activity in the supernatant was measured according to the
method described by Griffith (1980).
Results were defined as the units per milligram protein (U/mg protein).