Reagents
ACh chloride, (-)-nicotine tartrate, dimethylpiperazine (DMPP), cytisine, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), amphotericin B, penicillin/streptomycin, protease type XIV, collagenase Type I, poly-D-lysine hydrobromide, Red Blood Cell Lysis solution, dimethylsulfoxide, and all salts were purchase from Sigma-Aldrich (St. Louis, MO, USA). Varenicline tartrate was purchased from Tocris Bioscience (Abindgon, UK). Dulbecco’s Modified Eagle´s Medium (DMEM) and Glutamax were purchased from Life Technologies (Carlsbad, CA, USA). Fetal bovine serum (FBS) was from LabClinics (Barcelona, Spain) and the D-glucose from Panreac (Barcelona, Spain).