Reagents
ACh chloride, (-)-nicotine tartrate, dimethylpiperazine (DMPP),
cytisine, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES),
amphotericin B, penicillin/streptomycin, protease type XIV, collagenase
Type I, poly-D-lysine hydrobromide, Red Blood Cell Lysis solution,
dimethylsulfoxide, and all salts were purchase from Sigma-Aldrich (St.
Louis, MO, USA). Varenicline tartrate was purchased from Tocris
Bioscience (Abindgon, UK). Dulbecco’s Modified Eagle´s Medium (DMEM) and
Glutamax were purchased from Life Technologies (Carlsbad, CA, USA).
Fetal bovine serum (FBS) was from LabClinics (Barcelona, Spain) and the
D-glucose from Panreac (Barcelona, Spain).