Histology
Microscopic characteristic features showed three layers of the vaginal
outlet: superficial, intermediate, and deep, Fig. 2. The superficial
layer (the hymeneal membrane) of the vaginal outlet structure was the
mucosal type of tissue, Fig. 2. The epithelium that covers the hymen
membrane is a nonkeratinized striated squamous cell arranged in roses of
epithelial cells, on the average of three roses. The elastic lamina
propria consisted of a dense connective fibers layer projecting papillae
into the overlying epithelium with the capillary blood vessel network
and nerve endings, Fig. 2. Beneath the lamina propria was a strip of the
columnar epithelial cells. In addition, the parabasal, basal cells, and
basal lamina were identifiable. The intermediate-hymeneal ring was
composed of dense connective and muscle fibers, Fig. 2.
The deep layer of the vaginal outlet wall (the hymeneal plate) was
composed of longitudinal smooth muscles and connective tissues, Fig. 2.
This stratum was connected to the outer longitudinal vaginal wall
muscles.