Mitogenome sequencing, assembly and annotation
Illumina TruSeq libraries were prepared with an average insert size of 300 bp and sequenced on the Illumina NovaSeq 6000 platform (Beijing Berry Bioinformatics Technology Co., Ltd, China) generating 150 bp paired-end reads. The mitochondrial genome of typhlocybine species was assembled with NOVOPlasty v2.7.0 (Dierckxsens et al., 2017), using COI sequences (MN661136, MN699874, MG397188, KY039138, MT488436 and NC_046037) as seeds and the K-mer value set to 39. Annotation and visualization of the mitogenomes was accomplished with MitoZ v1.04 (Meng et al., 2019). Mitogenome sequences with low-quality assembly results were submitted to MITOS WebServer (Bernt et al., 2013), under default settings and the invertebrate genetic code. Gene boundaries were defined using the ARWEN v1.2 (Laslett & Canbäck, 2008), and Geneious Prime (Kearse et al., 2012). The 28 newly assembled mitogenome sequences were deposited in GenBank (Table S2).