Quantitative real-time PCR
Total RNA was extracted from frozen sinonasal mucosa by using isolation
kit ((DP431; Tiangen Biotech, Beijing, China). Total RNA was reverse
transcribed with the TaqMan Reverse Transcription Reagents Kit (Applied
Biosystems) based on the manufacturer’s instruction. Then, quantitative
real-time PCR analysis was performed on a Prism 7500 (Applied
Biosystems, Foster City, California) to evaluate the expression levels
of CP110, Foxj1 and Tap73. The relative fold increase of the three genes
expression was calculated by using the comparative
2-∆∆Ct methods, with the housekeeping gene
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the reference.