2.5 Phylogenetic analysis and sequence comparison
The σC genes of both newly
identified NDRV isolates were subjected to the online BLAST program
(https://blast.ncbi.nlm.nih.gov/Blast.cgi). And sequences sharing more
than 95% nucleotide identity were downloaded for further genetic
analysis with Clustal Omega. Furthermore, the phylogenetic tree was
conducted by MEGA 6.0 software with the neighbor-joining method using
1,000 bootstrap replicates.