Fig 2. Selection response in the acetates. Values shown are mean ± SEM. The control line is indicated in black, the high line in red (dotted) and the low line in blue (dashed). The dashed vertical line indicates the generation with adult females that developed from the last offspring that went through selection. A – C: Selection responses of the three acetates log-contrasted to 14:Ald. For the control line, 111 females were phenotyped in generation zero, 71 in generation one, and between 6 and 36 for the remaining generations. For the low and high line, respectively 189 – 296 and 189 - 295 females per generation were phenotyped. D: Difference in phenotypic means between selection lines and control in units of starting population standard deviation. The points and error bars with brighter hues show the average ± standard deviation for generation 1 – 3, 4 – 6, 7 – 10, and 11 – 13, respectively. E: Selection response of the relative total amount of acetates.
The other pheromone components showed no differentiation among selection lines or between selected and control lines (Fig 3A-C). The total amount of pheromone measured across the 11 biologically active components remained constant through time, indicating that changing ratios were independent of the pheromone titer (Fig 3D).