Sensory treatment
We first exposed 3 day-old wildtype (wt ) virgin males to females cues for 24 hours. To do so, we isolated standard males in a vial, and this vial was connected to either a) another vial containing three 3 day-old virgin wt females (i.e. female-exposed male) or b) to an empty vial (control male). Importantly, interconnected vials were separated by a fine mesh partition, and this allowed exchange of female semio-chemicals (volatiles but also probably non-volatiles) as well as female visual cues across the chambers, while ensuring males would not mate (GarcĂ­a-Roa, Serra and Carazo, 2018). Previous empirical research has determined that this methodology does not elicit any courtship behaviour in female-exposed males (Corbel et al. , 2022).