Figure Legends
Figure 1. Transcriptional analysis of Arabidopsis seedlings
co-cultivated with S. indica. A ) Venn diagram showing
the numbers of differentially expressed genes in Arabidopsis plants 2-
and 14-days post infection with S. indica compared to control
plants that were mock infected. B ) ClueGo analysis of induced
DEGs. The figure shows the representative molecular function interaction
among the targets. C ) qPCR analysis of transcriptional
responses of identified target genes in plants that were co-cultivated
for 2 and 14 days, respectively, with S. indica compared to mock
treated plants. The data represent means ± SE (n = 3).
Figure 2. Protein-protein interaction (PPI) studies to identify
binding partners of CBL7. A ) Yeast cells co-expressing the indicated
combinations of constructs were grown on non-selective (-LW) or
selective (–LWH) media. Colony growth on the selective media indicated
protein–protein interactions. The empty activation domain (AD) vector
was used as a negative control. Fivefold diluted cell cultures were
inoculated on each spot as indicated by the triangle widths. A
representative data set of an experiment replicated three times on
different biological samples is shown. BD, binding domain. B )
Interaction network for CBL7 inferred from 58 publicly available
datasets included in GeneMANIA v3.5.2. The PPI network indicates the
protein set that either directly (diamonds) or indirectly (circles)
interact with CBL7 (triangle). The different shape size refers to the
calculated interaction score, while the thickness of the edges indicates
the normalized maximal weight of the interaction.
Figure 3. Total root network length of Arabidopsis wild-type and
mutant plants after 14 days of co-cultivation with S. indica or
mock treatment . A ) Comparison of two cipk13 T-DNA
insertion mutant alleles with Col-0. B ) Analysis of the
fungus-triggered growth promoting effect in Col-3 and cbl7 mutant
plants. C ) The growth promoting effect of S. indica is
not affected in the hak5 mutant. The box plots show the median,
quartiles, and extremes of the compared datasets (n = 24). Asterisks
indicate significant differences between S. indica and mock treated
samples. Student’s t -test: **P≤0.01, ***P≤0.001.
Figure 4. Analysis of transcriptional differences betweencbl7 mutant and wild-type Col-3 plants. A ) DEGs
statistics for the cbl7 mutant compared to Col-3 control plants
under control conditions. B ) Volcano plot of the distribution
of all DEGs, mapping the 20 upregulated genes (red) and 53 downregulated
genes (blue). The figure shows the relation between the significance
(-log10(q-value)) and the strength
(log2FC) of the differential expression. C ) Bar
plot of the KEGG enrichment analysis of induced DEGs in cbl7compared to Col-3 control plants under control conditions. Only the
spliceosome KEGG term appeared to contain significantly enriched genes.
Gene enrichment is given by -log10(q-value).
Figure 5. K+ contents of root and shoot
tissues of S. indica infected and non-infected control plants .
The plants were grown in modified Hoagland medium under
K+ starvation (0 mM KCl) (A ) or at 1 mM KCl
(B ). The box plots show the median, quartiles, and extremes of
the compared datasets (n = 15). Different letters indicate significant
differences between the compared conditions and genotypes analyzed by
ANOVA and a Tukey-Kramer post hoc test (P<0.05).
Figure 6. Transcriptomics analysis of mock and S. indicatreated the cbl7 mutant plants to wild-type Arabidopsis.A ) DEGs statistics for the cbl7 mutant and Col-3 control
plants after co-cultivation with S. indica for 14 days.B ) KEGG enrichment analysis of induced DEGs in cbl7co-cultivated with the fungus versus similarly treated Col-3 plants.
Each circle in the figure represents a distinct KEGG pathway, and the
circle size indicates the number of genes enriched in the corresponding
metabolic pathway. The significance of the observed gene enrichment is
represented by a color gradient referring to the adjusted p-value
(padj). C ) Venn diagram showing the numbers of DEGs in Col-3
and cbl7 14-days post infection with S. indica compared to
control plants that were mock infected. D ) Functional network
analysis of DEGs in cbl7 and Col-3. Up- and down-regulated genes
are differentiated by color and shape. The shape size refers to the
different levels of differential expression according to thelog 2FC levels observed in the RNAseq analysis.
Functional associations are highlighted in the figure.
Figure 7. Analysis of the colonization of Col-3 and cbl7roots with S. indica . A ) Trypan blue stain of root
segments at 1 to 2 cm distance from the root tip. The figure shows
representative pictures for the three studied genotypes after 14 days of
co-cultivation with S. indica. B) Quantitative assessment of root
colonization by qPCR analysis of SiTEF1 . Depicted are means ± SE
of three independent biological experiments performed in triplicate. The
Arabidopsis GAPC2 gene served as endogenous control. Asterisks
indicate significant differences (Student’s t -test: ***P≤0.001).