Tenapanor treatment reverses cryptal hyperproliferation in the ileum and proximal colon
Assessment of the cryptal and villus length, the mucosal integrity and the muscle layer thickness was performed in all mice of the treatment groups. In the ileum, proximal and mid-distal colon, vehicle treatedCftr-/- mice displayed crypt villus/crypt elongation and increased muscle layer thickness compared to theCftr+/+ mice in the ileum and colon, but not the jejunum (Figure 4a-c). Treatment with tenapanor alleviated the crypt-villus/crypt elongation in the unobstructed ileum and proximal colon compared to the vehicle treated Cftr-/-mice (Figure 4b, two middle panels). The increase in muscle layer thickness, seen in all segments except the jejunum in theCftr-/- mice, was not significantly affected by tenapanor treatment. The jejunum did not display significant differences between the groups.