Tenapanor treatment reverses cryptal hyperproliferation in the
ileum and proximal colon
Assessment of the cryptal and villus length, the mucosal integrity and
the muscle layer thickness was performed in all mice of the treatment
groups. In the ileum, proximal and mid-distal colon, vehicle treatedCftr-/- mice displayed crypt villus/crypt
elongation and increased muscle layer thickness compared to theCftr+/+ mice in the ileum and colon, but not
the jejunum (Figure 4a-c). Treatment with tenapanor alleviated the
crypt-villus/crypt elongation in the unobstructed ileum and proximal
colon compared to the vehicle treated Cftr-/-mice (Figure 4b, two middle panels). The increase in muscle layer
thickness, seen in all segments except the jejunum in theCftr-/- mice, was not significantly affected by
tenapanor treatment. The jejunum did not display significant differences
between the groups.