Formulation and preparation
Galenic optimization of the topical formulation of rapamycin at 0.4% based on its excipients was performed. The most widely used classical formulation in the literature is petrolatum as a vehicle. However, other options were formulated based on previous studies.
Four diferent formulations were developed in biological safety cabinet, all of them with a rapamycin concentration of 0.4% and with differences in their excipients. The resulting formulations were: ointment, emulsion, gel and liposomes (Table 1). Each formulation was packaged in a bottle protected of ambient light and stored in a cold room at 5ºC±3ºC.