Rapamycin extraction
For the extraction of rapamycin from ointment, emulsion and gel
formulations 0.100 g of cream was introduced in a glass tube with 4 ml
of a mixture of acetonitrile, hexane and WFI and stayed in the vortex
for 10 seconds. The samples were then centrifuged at 3500 rpm for 10
minutes. The resulting supernatant was removed and 1 ml was aliquoted
for HPLC analysis. For the extraction of rapamycin from liposomes 0.100
g of cream was introduced in a glass tube with 4 ml of methanol. The
samples stayed in the vortex for 10 seconds, 50 μL was aliquoted and
after 1/20 dilution with methanol was analyzed with HPLC method.