Figure 10 Changes of intracellular NAD+/NADH
ratio in shake flask fed-batch LP and HP CHO cells under both constant
temperature and temperature downshift cultures. (●, black) temperature
downshift culture, (■, red) constant temperature culture. (a)
Time-series NAD+/NADH ratio in LP CHO cell; (b)
Significance analysis of NAD+/NADH ratio in LP CHO
cells on days 8-11; (c) Time-series NAD+/NADH ratio in
HP CHO cells; (d) Significance analysis of NAD+/NADH
ratio in HP CHO cells on days 8-11. Experimental values represent three
biological replicates ± SD. Two-tailed Student’s t-test was conducted
using GraphPad Prism 8.0 ((*) p < 0.05, (**) p <
0.01, (***) p < 0.001 and (****) p < 0.0001).