Intracellular CHO cell metabolite profiling and in vivo monitoring of redox state unravel the effect of temperature downshift on cell growth, antibody titer and product quality
Ziyu Zhu1, Xiaoqian Chen2, Wenhao Li1, Yingping Zhuang1,3, Yuzheng Zhao2,4 and Guan Wang1,3*
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai, People’s Republic of China.
2Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology (ECUST), Shanghai, People’s Republic of China.
3Qingdao Innovation Institute of East China University of Science and Technology, Shanghai, People’s Republic of China.
4Research Unit of New Techniques for Live-cell Metabolic Imaging, Chinese Academy of Medical Sciences, Beijing 100730, People’s Republic of China.
*Correspondence: Dr. Guan Wang (guanwang@ecust.edu.cn), State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People’s Republic of China. Tel: +86-21-64250719; Fax: +86-21-64253702
Running title: temperature downshift, redox state and antibody productivity