Intracellular CHO cell metabolite profiling and in vivo
monitoring of redox state unravel the effect of temperature downshift on
cell growth, antibody titer and product quality
Ziyu Zhu1, Xiaoqian Chen2, Wenhao
Li1, Yingping Zhuang1,3, Yuzheng
Zhao2,4 and Guan Wang1,3*
1State Key Laboratory of Bioreactor Engineering, East
China University of Science and Technology (ECUST), Shanghai, People’s
Republic of China.
2Optogenetics & Synthetic Biology Interdisciplinary
Research Center, State Key Laboratory of Bioreactor Engineering,
Shanghai Frontiers Science Center of Optogenetic Techniques for Cell
Metabolism, School of Pharmacy, East China University of Science and
Technology (ECUST), Shanghai, People’s Republic of China.
3Qingdao
Innovation Institute of East China University of Science and Technology,
Shanghai, People’s Republic of
China.
4Research Unit of New Techniques for Live-cell
Metabolic Imaging, Chinese Academy of Medical Sciences, Beijing 100730,
People’s Republic of China.
*Correspondence: Dr. Guan Wang (guanwang@ecust.edu.cn), State Key
Laboratory of Bioreactor Engineering, East China University of Science
and Technology, 130 Meilong Road, Shanghai 200237, People’s Republic of
China. Tel: +86-21-64250719; Fax: +86-21-64253702
Running title: temperature downshift, redox state and antibody
productivity