Figure 3 Determined concentrations of extracellular amino acid in CHO-K1 HP and CHO-K1 LP shake flask cultures. (●, black) LP constant temperature, (■, pink) HP constant temperature, (▼, green) LP temperature downshift, and (▲, purple) HP temperature downshift. The temperature was downshifted from 37°C to 33°C on day 5 (vertical dashed line). Samples were taken at 24h intervals and experimental values represent three biological replicates ± SD.