Figure 8 Intracellular reactive oxygen species (ROS) level of CHO-K1 HP and CHO-K1 LP under both constant temperature and temperature downshift cultures in shake flasks. (a) LP constant temperature culture. (b) LP temperature downshift culture. (c) Significance analysis of intracellular ROS percentage in LP CHO cells under both constant temperature and temperature downshift cultures on days 8-12. (d) HP constant temperature culture. (e) HP temperature downshift culture. (f) Significance analysis of the percentage of intracellular ROS in HP under both constant temperature and temperature downshift cultures on days 8-12. Experimental values represent three biological replicates ± SD. Two-tailed Student’s t-test was conducted using GraphPad Prism 8.0 ((*) p < 0.05, (**) p < 0.01, (***) p < 0.001 and (****) p < 0.0001)