Figure 11 Changes of intracellular NADPH level in shake flask fed-batch LP and HP CHO cells under both constant temperature and temperature downshift cultures. (●, black) temperature downshift culture, (■, red) constant temperature culture. (a) Time-series NADPH levels in LP CHO cells; (b) Significance analysis of NADPH levels in LP CHO cells on days 8-11; (c) Time-series NADPH levels in HP CHO cells; (d) Significance analysis of NADPH levels in HP CHO cells on days 8-11. Experimental values represent three biological replicates ± SD. Two-tailed Student’s t-test was conducted using GraphPad Prism 8.0 ((*) p < 0.05, (**) p < 0.01, (***) p < 0.001 and (****) p < 0.0001).
Table 1 Molecular size heterogeneity of antibodies in HP CHO cells. All bioreactor samples were subjected to a protein purification process. Molecular exclusion chromatography was used to determine high and low molecular species; non-reducing capillary electrophoresis was used to determine fragment content. High-molecular-weight species (HMW), low-molecular-weight species (LMW), and main peak (MP). Heavy-heavy-light chain (HHL).