DNA quantity and quality
All samples were quantified with either the dsDNA high sensitivity or broad range kits on the Qubit (Invitrogen, ThermoFisher). In the case of the chinook salmon, a TaqMan qPCR specific assay was used (Shelton et al., 2019) to quantify the amount of target DNA of each DNA extraction using gBlocksTM fragments (synthetic double stranded oligonucleotides from Integrated DNA Technologies Inc.) as standards from 105 to 1 copies µl-1 and assess the target to total ratio. The integrity of the DNA (DIN) was also evaluated using the Genomic DNA ScreenTape and kit on a 2200 TapeStation System (Agilent Technologies)