Plant material
The height Arabidopsis thaliana accessions were obtained from
INRA Versailles Genomic Resource Centre VNAT collection: An-1 (96AV,
Belgium), Bl-1 (42 AV, Italy), Col-0 (186AV, Poland), Cvi-0 (166AV, Cape
Verde), Ge-0 (101AV, Switzerland), Mt-0 (94AV, Libya), Oy-0 (224AV,
Norway) and Shahdara (Tadjikistan). Plants were grown in a growth
chamber under short photoperiod (8h light/16h dark), PPFD = 150 µmol
m-2 s-1, temperature 22°C/18°C and
relative humidity 60%. Plants were watered every 3 days and once a week
with Plant-Prod (15-10-30, N-P-K, Fertil). Fifty-five (55) days after
sowing, two fully developed leaves were sampled on each rosette and
instantly quenched in liquid nitrogen. Frozen samples were then
frozen-dried, dry material was grinded and 5 mg of dry powder used for
metabolites extraction by adding 400 µL of methanol/water (90/10) with
adonitol (55 µM) as an internal standard.