Parasitological examination
Skin samples were collected by scraping with a surgical blade, stored at 4°C and cultured in culture dishes at 37°C for two hours. The scrapings and cultures from skin samples were smeared on microscope slides with cedar wood oil and observed using light microscopy. Morphological characteristics, including the total size; morphology of the idiosoma, gnathosoma and legs; presence and characteristics of spines on the dorsal surface; and position of the anal plate, and taxonomic assignments were recorded(Samuel et al., 2001).