Metabolites, cell counts, and viability measurements
Cell culture supernatant was collected and analyzed for glucose (Glc), Lactate (Lac), pyruvate (Pyr), and lactate dehydrogenase (LDH) using Cedex Bio Analyzer (Roche) according to manufacturer’s instructions. Cell counts and viability were collected using NucleoCounter® NC-200 (ChemoMetec) according to manufacturer’s protocol.