Figure 2. Types and numbers of large repeat sequences in the
chloroplast genomes of five Primula species.
A total of 38 Microsatellites in the P. wilsonii chloroplast
genome were detected, including 28 mono-, five di-, two tri- and three
tetra-nucleotide repeats. In P. poissonii , 38 SSRs loci were
detected, consisting of 27 mono-, six di-, two tri- and three
tetra-nucleotide repeats, respectively (Table 2). Moreover, 41 SSRs loci
were spotted in P. secundiflora , and the numbers of different
types are listed in Table 2. Here, we found that the one-base SSRs loci
of P. wilsonii and P. poissonii were only A/T repeats. The
dinucleotide repeats were AT/TA in all the five Primula species.
It is shown that the trinucleotide repeats were AAT/ATT in P.
wilsonii , P. anisodora , P. miyabeana and P.
poissonii , but they were not present in P. secundiflora . The
tetra-nucleotide repeats AAAT/AATAwere present in all the Primulaspecies. However, the AAAG/CTTT and AATT repeats only appeared inP. secundiflora . Among these SSRs loci, 30 (78.95%) were in the
LSC region, six (15.79%) were in SSC region, and two (5.26%) were in
the IR region of the P. wilsonii chloroplast genome.
Table 2. Types and numbers of SSRs in the chloroplast genomes
of five Primula species.