3.1.2 External validation of the initial model
Lastly, the initial classification model –SVM, 3 biomarker peaks- (hyperparameter C: 0.19) developed with the strains characterized by WGS was blindly validated with the isolates characterized by ASO-PCR (n=32). The validation of the model yielded 90.0% correct classification of the strains, misclassifying only 3/32 ASO-PCR strains (two outbreak isolates as non-outbreak, and one non-outbreak as outbreak strain).