Genome-wide methylation evaluation with nanopore sequencing
data
The nanopore long-read data can be used to directly estimate epigenetic
modifications of nucleotide resolution [31]. The signals of cytosine
methylation were quantified with
Nanopolish
0.14.0 [65].
The
methylated sites and unmethylated sites were differentiated with the log
values of likelihood ratios > 2.5 and < -2.5,
respectively [118].