Genome-wide methylation evaluation with nanopore sequencing data
The nanopore long-read data can be used to directly estimate epigenetic modifications of nucleotide resolution [31]. The signals of cytosine methylation were quantified with Nanopolish 0.14.0 [65]. The methylated sites and unmethylated sites were differentiated with the log values of likelihood ratios > 2.5 and < -2.5, respectively [118].