Animals
Male Sprague-Dawley rats from CRIFFA (Barcelona; 200-250gr; 6-8 weeks) had free access to food pellets and tap water. They were maintained under the standard 12h dark/light cycle, with controlled temperature (22±2oC) and relative humidity (55-60%). All procedures concerned with housing, maintenance, and experimental treatment of the rats were approved by the Local Animal Ethics, Care, and Use Committee for the University of Málaga, Spain. Guidelines for animal experiments were carried out following EU Directive 2010/63/EU and Spanish Directive (Real Decretory 53/2013) recommendations.