2.2. Protein 3D modeling
The protein 3D models were generated with RaptorX Contact Prediction Server 22. Superposition between each model and the templates was done using the align command in PyMOL 2.3.1 version {The PyMOL Molecular Graphics System}. The regions implicated in substrate binding and activity were manually annotated using the pattern sequences or 3D structure of cellulase templates available in Prosite and RCSB Pdb database (rcsb.org 23).