2.2. Protein 3D modeling
The protein 3D models were generated with RaptorX Contact Prediction
Server 22. Superposition between each model and the
templates was done using the align command in PyMOL 2.3.1 version {The
PyMOL Molecular Graphics System}. The regions implicated in substrate
binding and activity were manually annotated using the pattern sequences
or 3D structure of cellulase templates available in Prosite and RCSB Pdb
database (rcsb.org 23).