Abstract
Indoxyl is an intermediate product in the production of indigo and
indirubin. There are no studies on the inhibitory effect of indoxyl on
microorganism. In this study, it was found that temperature-stress
induced the synthesis of intracellular β-glucosidase, which had a great
influence on the ratio of indican, indoxyl, indigo and indirubin inStrobilanthes cusia . At 100 ℃, only indican was detected, whereas
a large amount of indoxyl was found at 50–70 ℃. HPLC analysis indicated
that the use of indoxyl reduced glucose consumption and metabolite
production by Staphylococcus aureus . The cells treated with
indoxyl had irregular shapes, and fragments of cells were found in SEM
images. The images of CLSM suggest that cell morphological changes would
be caused by the inhibition of indoxyl on EMs formation. Moreover,
indoxyl decreased intracellular ATP content and increased the
NAD+/NADH ratio, which promoted the generation of
H2O2, damaging cells.