2.12 Hair cycle analyses
The analyses of hair cycle were centered on level of skin pigmentation and hair shaft growth, as earlier reported (Stenn & Paus, 2001). Briefly, clean-shaved hair-coat-recovery values were premeditated based on skin pigmentation levels from 0 to 100% (Chai et al., 2019). For illustration, 0% implied no hair growth (and no pigmentation); 50% couched full-length just grown hairs casing half of the dorsal designated skin (or pigmentation level on 100% of the dorsal definite skin area without any hair shafts); 70% implicit full-length-hair progress on ~70% of the dorsal skin (or pigmentation level covering 100% of dorsal skin along with 30–40% hair shafts); 100% inferred afresh full-length hair growth.
2.13 Hair follicles count
The H&E stained slides were photographed using a digital photomicrograph and all of the images were cropped in a fixed width of 300 pixels. We counted hair follicles manually in a fixed area (0.09 mm2). Digital photomicrographs were taken from representative areas at a fixed magnification of 100X.