2.12 Hair cycle analyses
The analyses of hair cycle were centered on level of skin pigmentation
and hair shaft growth, as earlier reported (Stenn & Paus, 2001).
Briefly, clean-shaved hair-coat-recovery values were premeditated based
on skin pigmentation levels from 0 to 100% (Chai et al., 2019). For
illustration, 0% implied no hair growth (and no pigmentation); 50%
couched full-length just grown hairs casing half of the dorsal
designated skin (or pigmentation level on 100% of the dorsal definite
skin area without any hair shafts); 70% implicit full-length-hair
progress on ~70% of the dorsal skin (or pigmentation
level covering 100% of dorsal skin along with 30–40% hair shafts);
100% inferred afresh full-length hair growth.
2.13 Hair follicles count
The H&E stained slides were photographed using a digital
photomicrograph and all of the images were cropped in a fixed width of
300 pixels. We counted hair follicles manually in a fixed area
(0.09 mm2). Digital photomicrographs were taken from
representative areas at a fixed magnification of 100X.