2.11 Histology and immunohistochemistry analyses
Samples were immobile in 4% formaldehyde in PBS for 24 h, then
dehydrated and implanted in paraffin, sectioned at 5 μm before
haematoxylin and eosin (H&E) staining and immunohistochemistry were
accomplished. The primary antibodies exploited for immunohistochemistry
include anti‐Ki‐67 (1:200, Cell Signaling Technology Cat# 12202,
RRID:AB_2620142), anti‐LGR5 (1:100, Abcam Cat# ab75732,
RRID:AB_1310281), Anti-active β-catenin antibody (1:100 Cell Signaling
Technology Cat#8814, RRID:AB_11127203) and anti‐β‐catenin (1:100 Cell
Signaling Technology Cat# 9562, RRID:AB_331149).