3.7 ISX9 promotes the expression of stemness marker genes SOX2,
LGR5, Twist1, and OCT4
Concerning the importance of the Wnt/β-catenin pathway in the
self-renewal and expansion of various stem cell populaces (Ring, Kim, &
Kahn, 2014; Tao et al., 2020), the expression of stemness marker genes
SOX2, LGR5, Twist1, and OCT4 was examined after treatment with ISX9 by
real-time PCR and immunoblotting. As seen in Figure 5, ISX9 treatment
significantly enhanced the mRNA of SOX2, LGR5, Twist1, OCT4, and protein
levels of SOX2, LGR5, and Twist1 in HEK293T, HaCat and NIH3T3 cells
(Figure 5G-L), suggesting that ISX9 may have a potential to promote the
stemness property via activating the Wnt/β-catenin pathway.