SUPPLEMENTAL FIGURES
Figure S1. Treatment with ISX9 does not affect the mRNA
expression of β-catenin in HEK293T and HaCat cells. (A) HEK293T and (B)
HaCat cells were treated with the indicated concentrations of ISX9 for
24 h. The relative mRNA level of β-catenin was measured by real-time
PCR. The data from three independent experiments are presented.
Figure S2. ISX9 inhibits the interaction of Axin1 with
β-catenin. HEK293T cells were incubated with the indicated
concentrations of ISX9 for 24 h. Cell lysates were extracted and
subjected to immunoprecipitation (IP) using anti-β-catenin antibody
followed by Western blotting using specific antibodies as indicated.