TXA Analysis
Analysis of TXA was conducted using an HPLC assay based on the British Pharmacopoeia (2019) method. Briefly, chromatography was performed on a Shimadzu Nexera UHPLC system using an Agilent Zorbax Eclipse Plus C18 column (4.6 x 250 mm, 5 µm particle size). The mobile phase comprised 0.1 M anhydrous sodium dihydrogen phosphate dissolved in 60:40 water:methanol and adjusted to pH 2.5 with phosphoric acid. Sodium dodecyl sulphate (SDS, 5 mM) was added as an ion pairing reagent and separation was performed in isocratic mode at a flow rate of 0.9 mL/min using a 20 µL injection volume. TXA for injection samples (100 mg/mL) were diluted 1:20 to bring them within the validated range of the method (2.5 – 6.0 mg/mL).