RESULTS
Mean values of selected blood parameters according to sample timing are
shown in Table 2. Using a MANOVA analysis, significant differences
between first and second samples and between sexes were found, but there
was no significant interactive effect between sample and sex (Table 3).
Significant differences between the two samples were found in most of
the parameters (Table 2). Among hematological parameters, all showed
significant differences early and later in hacking, including red blood
cells, lymphocytes and monocytes. Hemoglobin, PCV and thrombocytes
decreased during captivity, but white blood cells and most related
component metric values increased. All the biochemical parameters, apart
from AST and LDH, showed significant differences (Table 2). Albumin and
globulin increased as did cholesterol, calcium and potassium.
Nitrogenous components, urea or uric acid, decreased together with
glucose and sodium. Weights (body mass) of the nestlings varied
significantly, being lower in the second sample. This decrease in body
mass was similar for both sexes (Fig. 1).
For urea, the form of the distribution also changed from a skewed
(quasi-Poisson) distribution in the sample when entering the hacking
facility (Shapiro-Wills = 0.889, P < 0.001), to a normal
distribution of values after their stay in the hacking facility
(Shapiro-Wills = 0.965, P = 0.246, Fig. 2). A significant linear
regression between standardized hatching date and urea level was found
only for the first samples (r = 0.396, R2 = 0.16, P =
0.010), but not for the second samples, after their stay in captivity (r
= 0.297, R2 = 0.08, P = 0.059) (Fig. 3).