FIGURE LEGENDS
Figure 1 . Principle component analysis (A-C) and orthogonal
parti al least squares discriminant analysis (D-F) scores plots
comparing urine samples from no AKI (black) and AKI (red) patients at
each timepoint: (A, D) pre, (B, E) 24-48h, and (C, F) post. Model
statistics for OPLS-DA were as follows: (D) pre: R2Y = 0.784, Q2Y =
0.389 (E) 24-48h: R2Y = 0.752, Q2Y = 0.384 (F) post: R2Y = 0.969, Q2Y =
0.700. n=18-20 for no AKI patients and n=10-11 for AKI patients.
Figure 2. Principle component analysis (A-C) and orthogonal
partial least squares discriminant analysis (D-F) scores plots comparing
serum samples from no AKI (black) and AKI (red) patients at each
timepoint: (A, D) pre, (B, E) 24-48h, and (C, F) post. Model statistics
for OPLS-DA were as follows: (D) pre: R2Y = 0.991, Q2Y = 0.668 (E)
24-48h: R2Y = 0.776, Q2Y = 0.317 (F) post: R2Y = 0.995, Q2Y = 0.813.
n=18-20 for no AKI patients and n=10-11 for AKI patients.
Figure 3 . (A-D ) Log transformed relative intensity of
urine features significantly different between no AKI and AKI patients
at the pre timepoint. Data are presented as box and whisker plots, where
boxes represent the median and interquartile range (IQR), and whiskers
represent 1.5x IQR. (F) Diagnostic performance of urine
metabolites as assessed by the area under the receiver operating curve.
Statistical analysis was performed using two-way ANOVA,
*p<0.05, **p<0.01 compared to no AKI patients at
each timepoint. n=18-20 for no AKI patients and n=10-11 for AKI
patients.
Figure 4. (A-J ) Log transformed relative intensity of
urine features significantly different between no AKI and AKI patients
at the post timepoint. Data are presented as box and whisker plots,
where boxes represent the median and interquartile range (IQR), and
whiskers represent 1.5x IQR. (K) Diagnostic performance of
urine metabolites as assessed by the area under the receiver operating
curve. Statistical analysis was performed using two-way ANOVA,
*p<0.05, **p<0.01, ***p<0.001 compared to
no AKI patients at each timepoint. n=18-20 for no AKI patients and
n=10-11 for AKI patients.
Figure 5. (A-G ) Log transformed relative intensity of
serum features significantly different between no AKI and AKI patients
at the post timepoint. Data are presented as box and whisker plots,
where boxes represent the median and interquartile range (IQR), and
whiskers represent 1.5x IQR. (H) Diagnostic performance of
urine metabolites as assessed by the area under the receiver operating
curve. Statistical analysis was performed using two-way ANOVA,
*p<0.05, **p<0.01, ***p<0.001 compared to
no AKI patients at each timepoint. n=18-20 for no AKI patients and
n=10-11 for AKI patients.
Figure 6. Log transformed relative intensity of urine and serum
features significantly altered over time in no AKI patients. Data are
presented as box and whisker plots, where boxes represent the median and
interquartile range (IQR), and whiskers represent 1.5x IQR. Statistical
analysis was performed using one-way ANOVA, *p<0.05,
**p<0.01, ***p<0.001 compared to saline control at
each timepoint, n=18-20.