2. METHODS
In our study; A total of 45 patients (33 men, 12 women) who were taken to cardiopulmonary bypass due to open heart surgery in the Cardiovascular Surgery department were selected. Group 1 patients were defined as undergoing coronary artery bypass surgery (25 patients), and Group 2 patients as undergoing coronary artery bypass+mitral insufficiency surgery (20 patients). Preoperative (preop) and postoperative (postop) blood samples of these patients were studied. The study was initiated with the approval of the Harran University Ethics Committee. Patients under 18 years of age, chronic kidney and liver failure, patients with low ejection fraction, additional valve disorders, long-term use of angiotensin receptor blockers and aldosterone antagonists, chronic rheumatic disease, aortic aneurysm and collagen tissue disease were excluded from the study. Standard anesthesia protocol, venous and coronary sinus blood were taken. After aortic and venous cannulation after sternotomy in the patients included in the study, operations were completed at 28-32 °C under cardiopulmonary bypass and cross-clamp.
Fresh blood collected in heparinized tubes was centrifuged at 5000 rpm for 10 minutes and their plasmas were separated. The separated plasmas were placed in Eppendorf tubes and kept at -80 degrees. On the working day, these bloods were thawed and studied. The unit of measurement used is U / L.
Statistical analysis
Statistical analyzes were performed using the SPSS® Version 16.0 (SPSS Inc. Chicago USA)® computer program. The significance of the difference between the means of the groups was compared with the One-Way ANOVA test. Values less than p < 0.05 were considered statistically significant.