2. METHODS
In our study; A total of 45 patients (33 men, 12 women) who were taken
to cardiopulmonary bypass due to open heart surgery in the
Cardiovascular Surgery department were selected. Group 1 patients were
defined as undergoing coronary artery bypass surgery (25 patients), and
Group 2 patients as undergoing coronary artery bypass+mitral
insufficiency surgery (20 patients). Preoperative (preop) and
postoperative (postop) blood samples of these patients were studied. The
study was initiated with the approval of the Harran University Ethics
Committee. Patients under 18 years of age, chronic kidney and liver
failure, patients with low ejection fraction, additional valve
disorders, long-term use of angiotensin receptor blockers and
aldosterone antagonists, chronic rheumatic disease, aortic aneurysm and
collagen tissue disease were excluded from the study. Standard
anesthesia protocol, venous and coronary sinus blood were taken. After
aortic and venous cannulation after sternotomy in the patients included
in the study, operations were completed at 28-32 °C under
cardiopulmonary bypass and cross-clamp.
Fresh blood collected in heparinized tubes was centrifuged at 5000 rpm
for 10 minutes and their plasmas were separated. The separated plasmas
were placed in Eppendorf tubes and kept at -80 degrees. On the working
day, these bloods were thawed and studied. The unit of measurement used
is U / L.
Statistical analysis
Statistical analyzes were performed using the SPSS® Version 16.0 (SPSS
Inc. Chicago USA)® computer program. The significance of the difference
between the means of the groups was compared with the One-Way ANOVA
test. Values less than p < 0.05 were considered statistically
significant.