Fig.4 Application of Cas13b(d) in endogenous gene knock-down. (A) Knock-down of Zeb1 RNA expression by CRISPR/Cas13b. (B) Knock-down of Zeb1 RNA expression by CRISPR/Cas13d. (C-D) Knock-down of Zeb1 RNA expression by CRISPR/Cas13 with gRNA at different position of target RNA. (E-F) Knock-down of Zeb1 RNA expression by CRISPR/Cas13 with different length of gRNA. (G) The scheme shows that 3 gRNAs targeting Dnmt3a are produced with three independent gRNA with one plasmid in precursor manner. (H) Knock-down ofDnmt3a RNA expression by CRISPR/Cas13 with mixed plasmids of gRNA or gRNAs precursor plasmid. (I) The scheme shows that gRNA 1-3 targetingDnmt3a , PBXIPI , and Zeb1 are produced with one precursor plasmid. (J-L) Knock-down of RNA expression of Dnmt3a ,PBXIPI , and Zeb1 simultaneously by CRISPR/Cas13 with mixed plasmids of gRNAs or precursor plasmid.