Generation of CRIPSR/Cas13 mice.
All systemic CRISPR/Cas13 expression mice were generated using the
CRISPR/Cas9 technique in Shanghai Model Organisms Center, Inc (Shanghai,
China). Briefly,a mixture of gRNA targeting Rosa26, Cas9 mRNA and Cas13
donor plasmid with 3.0 kb homologous arm was microinjected into one-cell
stage mouse embryos from C57BL/6 mice. The injected embryos were then
implanted into pseudo-pregnant C57BL/6 mice to generate F0 mice. The F0
mice were genotyped using PCR. The heterozygote mice were bred at the
Shanghai Model Organisms Center prior to starting any experiments. The
breeding and feeding were conducted according to Institutional Animal
Care and Use Committees protocols.