Fig.5 Off-target Effects of Cas13b and Cas13d. (A-D) Volcanic map of gene fold change, b20 (A), b30 (B), d20 (C), d30 (D) vs NC. The results were presented as mean ± SD. Data are analyzed by one-way ANOVA. ****P < 0.0001. (E) Luciferase expression in 293T cells after a 48-hours transfection, renilla luciferase activity is used as a reference. (F-G) The numbers of differential genes among the four groups. Different colored boxes represent different level of fold change, and each column represents the total number of differential genes. (H-I) Venn diagram shows differential genes overlapped among the four groups of samples. (J-M) Arrangement of GO pathways enrichment score. BP: Biological Process; CC: Cellular Component. MF: Molecular Function.