Fig.4 Application of Cas13b(d) in endogenous gene knock-down. (A)
Knock-down of Zeb1 RNA expression by CRISPR/Cas13b. (B)
Knock-down of Zeb1 RNA expression by CRISPR/Cas13d. (C-D)
Knock-down of Zeb1 RNA expression by CRISPR/Cas13 with gRNA at
different position of target RNA. (E-F) Knock-down of Zeb1 RNA
expression by CRISPR/Cas13 with different length of gRNA. (G) The scheme
shows that 3 gRNAs targeting Dnmt3a are produced with three
independent gRNA with one plasmid in precursor manner. (H) Knock-down ofDnmt3a RNA expression by CRISPR/Cas13 with mixed plasmids of gRNA
or gRNAs precursor plasmid. (I) The scheme shows that gRNA 1-3 targetingDnmt3a , PBXIPI , and Zeb1 are produced with one
precursor plasmid. (J-L) Knock-down of RNA expression of Dnmt3a ,PBXIPI , and Zeb1 simultaneously by CRISPR/Cas13 with mixed
plasmids of gRNAs or precursor plasmid.