Figure 3 Detection of S1-specific ASCs in spleen and lung tissues.
Mice were triple vaccinated by S1-IM, S1-AS03-IM and S1-SF-10-IT every two weeks. At two weeks after the last immunization, the spleen and lungs were dissected out and lymphocytes isolated. The latter were seeded onto S1-coated plates at 2 × 105 (lungs) or 106 (spleen) cells, and S1-specific IgG ASCs in spleen (A), IgA ASCs in spleen (B), IgG ASCs in lungs (C) and IgA ASCs in lungs (D) were detected by ELISPOT assay. Each bar represents mean ± SEM counts of ASC per 106 cells (n = 10). Differences between groups were analyzed by the non-parametric Mann–Whitney U-test.*P <0.05,**P <0.01 vs S1-IM,††P <0.01 vs S1-AS03-IM.