Figure 4 Detection of S1-responsive CSCs in spleen and lung tissues.
Mice were triple vaccinated by S1-IM, S1-AS03-IM and S1-SF-10-IT every two weeks. At two weeks after the last immunization, lymphocytes from the spleen and lungs of immunized mice (n = 10 mice per group) or untreated negative control mice (n = 3) were isolated, and then seeded and incubated with or without 20 μg/mL of S1 for 24 h. The IFN-γ (A, B), IL-4 (C, D) and IL-17A (E, F) CSCs in lymphocytes of spleen (A, C, E) and lungs (B, D, F) were detected by ELISPOT. Data are mean ± SEM counts of non-stimuli well (open bars) and S1-stimuli well (closed bars) of CSC per 106 cells (n = 10). Differences between groups were analyzed by the non-parametric Mann–Whitney U-test.*P <0.05,**P <0.01 vs S1-IM,††P <0.01 vs S1-AS03-IM.