Aquatic vegetation alters the hydrodynamics of natural waters, such as rivers, lakes, and estuaries. Plants can generate turbulence that propagates throughout the entire water column, which affects gas transfer mechanisms at both air-water and water-sediment interfaces, driving changes of dissolved oxygen (DO), an important indicator of water quality. We conducted a series of laboratory experiments with rigid cylinder arrays to mimic vegetation using a staggered configuration in a recirculating race-track flume. Walnut shells were chosen as the sediment substrate, which interacts with DO in water. 2D planar Particle Image Velocimetry was used to characterize the flow field under various submergence ratios, highlighting the effect of vegetation on turbulence quantities. Gas transfer rates were determined by measuring the DO concentration during the re-aeration process based on the methodology proposed by the American Society of Civil Engineers. Our data provide new insight on Air-Water-Vegetation-Sediment interactions in streams as a function of submergence ratio, array density, and flow turbulence. A modified surface renewal model using turbulence production as an indicator of gas transfer efficiency is used to predict surface gas transfer rates. A delayed time of re-aeration between the bulk and the near-bed region was observed and varies with flow velocities and submergence ratios, which controls the oxygen flux from water to sediment. Future studies are required to investigate the cause of the delayed time to incorporate sediment oxygen demand in a substrate-to-surface transfer model.