Bioanalyses
The biological sample analysis test will be blinded, where the
administered formulation is not known for each cycle during the sample
analysis. Appropriate sample pretreatment methods were established based
on drug properties, and a validated liquid chromatography-tandem mass
spectrometry (LC-MS/MS) method was used to determine the blood
concentration of nifedipine. The internal standard method (internal
standard nifedipine d6) was used for quantification and the sample
pretreatment method as protein precipitation.