Figure 7 The effect of CD40-CD40L and ICOS-ICOSL pathways on eosinophil activation
Peripheral blood of ECRS patients was stimulated with medium alone (Control, circle), IgG (5ug/mL, rectangle), rhCD40L (5ug/mL, triangle) or rhICOS (10ug/mL, triangle) for 24h. Then CD69 expression on eosinophils was detected by Flow cytometry. (A). The gating strategy of activated eosinophils (CD45+CD16-CD69+). (B). The percentage of activated eosinophils after different stimulation.