Figure 8 The effect of TNF-α and IL-5 on CD40 expression of
purified eosinophils
GM-CSF (50 ng/mL) was added as a basic condition (medium) to keep
eosinophils culture for all groups. Freshly isolated eosinophils from
healthy controls were cultured with medium alone, or rhTNF-α (50 ng/mL),
or rhIL-5 (50 ng/mL) or rhTNF-α plus rhIL-5 in the absence or presence
of specific p38 inhibitor SB203580 (10 uM) for 24h and 48h. And SB203580
was preincubated with eosinophils for 1h before cytokines stimulation.
CD40 expression was subsequently detected by Flow cytometry.