Figure 1: Utilisation of single-cell RNA-seq to study
baculovirus-infected Sf9 insect cells during AAV2 production. a)
Overview of the cell culture and infection strategy. b) Viable cell
concentration (•) and cell viability (o) throughout infection. The
arrows show timepoints captured for scRNA-seq analysis. c) Intracellular
genomic AAV particles (VG.mL-1) along infection (n=1).
d) Percentage of mitochondrial UMIs per cell for all samples. The red
dashed line indicates the quality filter threshold applied in this study
(percentage of mitochondrial genes < 5% considered for
further analysis). e) Number of cells that have passed (i.e., considered
valid) or have been removed in the quality filtering step in each
sample. f) Total genes detected per cell. A bimodal distribution can be
observed for the 24 hpi sample, with 21% of cells having less than 2000
genes detected while being associated with a high percentage of
baculovirus UMIs.