Figure 1: Utilisation of single-cell RNA-seq to study baculovirus-infected Sf9 insect cells during AAV2 production. a) Overview of the cell culture and infection strategy. b) Viable cell concentration (•) and cell viability (o) throughout infection. The arrows show timepoints captured for scRNA-seq analysis. c) Intracellular genomic AAV particles (VG.mL-1) along infection (n=1). d) Percentage of mitochondrial UMIs per cell for all samples. The red dashed line indicates the quality filter threshold applied in this study (percentage of mitochondrial genes < 5% considered for further analysis). e) Number of cells that have passed (i.e., considered valid) or have been removed in the quality filtering step in each sample. f) Total genes detected per cell. A bimodal distribution can be observed for the 24 hpi sample, with 21% of cells having less than 2000 genes detected while being associated with a high percentage of baculovirus UMIs.