Results
Screening of plant extracts for their potential sPLA2 inhibitory activity
We initially compared the sPLA2 inhibitory activities of the different extracts of T. hispida (THA, THE and THB), C.rotundus (CRA and CRE) and J.adathoda (JAA and JAE) with that of a commercial sPLA2 inhibitor (CAY 10590) on bee venom. Bee venom is known to have high levels of sPLA2 IIA. The inhibitory activity of each extract at different concentrations on bee venom sPLA2 is shown in table 1. We found that the extracts of T. hispida (THA and THE), showed the highest sPLA2 activity, which was comparable to the inhibition levels of CAY 10590. Of the T. hispida extracts, THB showed the highest level of inhibition of the sPLA2 activity and therefore, we proceeded with further analysis of this extract.