Assays for measuring secretory phospholipase A2 (sPLA2) activity
and sPLA2 inhibitors
The sPLA2 activity was determined by a commercial assay (Abcam, UK),
containing bee venom as the positive control (Catalog no: ab133089). The
assay was carried out and interpreted according to the manufacturer’s
instructions. All assays were carried out in triplicate. The sPLA2
activity was expressed as
nmolmin-1mL-1. The sPLA2 inhibitory
activity of plant extracts was compared with the commercially available
sPLA2 inhibitor, CAY10590 (Cayman, USA).