2.5 | Bioinformatics analysis
To obtain high-quality clean reads, raw reads were demultiplexed and
filtered for quality based on the methods developed by Fadrosh et al.
(2014).
Cleaned
tags were obtained by FASTP (Chen et al., 2018). Sequences were assigned
to operational taxonomic units (OTUs) based on a similarity cut-off of
97% by using UPARSE (v7.0.1090) (Edgar, 2013). The representative
sequences from each OTU were then taxonomically classified using the
Ribosomal Database Project (RDP) Classifier (v2.2) with confidence
threshold of 80%.