Ionomic analysis
Root ionome was assessed in plants submitted for 10 days to treatment
conditions. Roots were carefully washed with 18 MΩ to remove nutrients
from solution. Plant material was dried for 4 days at 60 ºC.
Approximately 0.1g was used to perform an open-air digestion in Pyrex
tubes using 0.7 mL concentrated HNO3 at 110 ºC for 5 h
in a hot-block digestion system (SC154-54-Well Hot Block™, Environmental
Express, SC, USA) placed inside a flow cabinet. The concentrations
of selected elements (Ca, K, Mg, Na, P, S, B, Mo, Cu, Fe, Mn, Zn) were
determined by inductively coupled plasma optical emission spectroscopy
ICP-OES (Thermo Jarrell-Ash, model 61E Polyscan, England).